Aerotek Scientific is actively recruiting for a Cell Culture Biologist with excellent organizational, problem solving, and communication skills to join a biopharmaceutical company located in Boulder, Colorado. This scientist will be responsible for maintaining and scaling up ( ? 5L) insect cell and E. coli cultures in suspension for recombinant protein expressions. The individual will also be involved in molecular cloning and construct design. A track record of excellent aseptic techniques and experience in molecular biology and protein expression are essential for this role. Responsibilities: Passage suspended insect cell cultures.Generate baculovirus stocks for expressing recombinant proteins in insect cells.Evaluate and optimize protein expression in insect cells and E. coli.Coordinate and prioritize insect cell protein expression queues for the Structural Biology group. Document and maintain records on constructs, strains, and protein expressions.Multi-liter scale of protein expressions in insect cells and E. coli.Perform molecular cloning and DNA preparation for transfection.Requirements: BS or MS in Molecular Biology, Cell Biology, Biochemistry or related biological science with minimum 1 year experience in maintaining cell cultures within an industrial or academic research team (cell culture experience from a lab course does not qualify).Experience in E. coli transformation, insect or mammalian cell transfection, and protein expression in these hosts.Experience with WAVE bioreactor or construct design for protein expression is highly desirable.Able to lift 50lb objects with dimensions 12 inches in height and 26 inches in width.Able to perform molecular cloning, PCR, gel electrophoresis for DNA and protein analysis, and immunoblotting.Familiar with Microsoft Office; advanced user of Access is a plus. Able to work independently yet cooperatively; enthusiastic about learning new biology and techniques, and readily apply those learnings to immediate challenges.Please apply with two professional reference and an updated resume.About Aerotek: We know that a company's success starts with its employees. We also know that an individual's success starts with the right career opportunity. As a Best of Staffing Client and Talent leader, Aerotek's people-focused approach yields competitive advantage for our clients and rewarding careers for our contract employees. Since 1983, Aerotek has grown to become a leader in recruiting and staffing services. With more than 250 non-franchised offices, Aerotek's 8,000 internal employees serve more than 300,000 contract employees and 18,000 clients every year. Aerotek is an Allegis Group company, the global leader in talent solutions. Learn more at .The company is an equal opportunity employer and will consider all applications without regards to race, sex, age, color, religion, national origin, veteran status, disability, sexual orientation, gender identity, genetic information or any characteristic protected by law.If you would like to request a reasonable accommodation, such as the modification or adjustment of the job application process or interviewing process due to a disability, please call 888-###-#### or email accommodation@aerotek .com for other accommodation options. However, if you have questions about this position, please contact the Recruiter located at the bottom of the job posting. The Recruiter is the sole point of contact for questions about this position. Associated topics: biological engineer, biomaterials, biomechanics, biomedical, bioprocess, fermentation, neuro, neurodegenerative, pathogenesis, therapeutic
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