Employment Status: AF - Active - Regular - Full Time
We are in the process of Visualizing and measuring the unfolded protein response in primary cells.
The unfolded protein response (UPR) is a universal stress response that, remarkably, is used in a variety of responses to metabolic changes, such as rapid changes in glucose or oxygen levels due to diet or exercise, respectively. The UPR is also used to respond to bacterial or viral infections. Each of such conditions alters the protein synthesis machinery of the affected cells, an alteration that is detected through the UPR sensors IRE1, PERK and ATF6. The activation of these sensors, either singly or together, leads to a set of responses that initiate a set of molecular coping mechanisms to restore the cells to their original equilibrium.
We have made a variety of fluorescent proteins that are activated in cells undergoing the UPR and are using them to visualize the response of primary cells (pancreatic beta cells. neurons or glial cells) to metabolic changes and quantify the response. The student will participate in live imaging of the cells and in analyzing the recorded responses. The goal is to determine how the UPR changes with increased level of metabolic stress (e.g. ranges of glucose fluctuations, lipotoxic agents or hypoxic levels) and how the graded response relates to the outcome of the coping response.
* Performs lab tests which require the analysis of results and interpretation of methods
* Performs research procedures on experimental samples as directed
* Assists with planning specific research protocols and procedures
* Assists in development & troubleshooting of new techniques and ideas
* Modifies methods and protocols under the direction of the PI
* Formulates and performs complex lab experiments and analyzes & reports the data
* Presents & discusses work at weekly lab meetings
* Prepares laboratory reagents and monitors and maintains inventory
* Performs calculations and maintains appropriate log books and lab notebooks
* Assists in writing methods and setting-up protocols accurately under the direction of the program director or senior scientist
* Maintains laboratory equipment, safety procedures and compliance records
* Researches journal, white papers and other scientific publications for pertinent information
* Assists with the preparation of data for publication or presentation at symposia
* May supervises junior members of laboratory.
Required Education and Experience
* Bachelor's degree, 3-5 years scientifically relevant experience
Additional Technical Requirements
* Proficient in various research and/or analytical techniques required
All CHOP employees who work in a patient building or who provide patient care are required to receive an annual influenza vaccine unless they are granted a medical or religious exemption.
Children's Hospital of Philadelphia is committed to providing a safe and healthy environment for its patients, family members, visitors and employees. In an effort to achieve this goal, employment at Children's Hospital of Philadelphia, other than for positions with regularly scheduled hours in New Jersey, is contingent upon an attestation that the job applicant does not use tobacco products or nicotine in any form and a negative nicotine screen (the latter occurs after a job offer).
Children's Hospital of Philadelphia is an equal opportunity employer. We do not discriminate on the basis of race, color, gender, gender identity, sexual orientation, age, religion, national or ethnic origin, disability or protected veteran status.
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