The goal of the Allen Institute for Cell Science is to understand cell behaviors in their normal, pathological, and regenerative contexts. The Institute, located in a new facility on South Lake Union in Seattle, Washington, will employ a multi-disciplinary team approach to generate novel cellular reagents, models, and databases that are informed by and open to the community. The Institute aims to understand cells, individually and in collectives, as integrated systems of organelles and molecular machines that are regulated by signaling complexes, determining how they integrate to generate the plethora of observed cellular behaviors. It will do this using human induced pluripotent stem cells (hiPSc), genome editing, a large live cell microscopy pipeline, predictive modeling and theory, and creating a novel multi-scale, dynamic and visual output for the experiments and models.
This specialist will help with the design, setup, and maintenance of the microscopy pipeline. The pipeline uses multiple microscopy modalities (e.g. widefield microscopy, spinning disk confocal microscopy, super-resolution microscopy). The successful candidate will have to be able to specify and assemble components of the pipeline (e.g. cameras, objectives, laser lines). The position requires troubleshooting of the microscopes and coordination with vendor service and other third-party contributors. Experience in the implementation and application of advanced technologies like fluctuation methods (e.g. FCS, pair-correlation) and FRAP are beneficial. The candidate will be involved in developing SOPs and training of staff. Candidates with industry background are highly encouraged to apply.
To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. The requirements listed below are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.
* Perform maintenance and troubleshooting of microscopes
* Assist with the setup of the microscopy pipeline
* Specify components for the pipeline
* Coordinate with vendors and other third parties
* Perform advanced imaging tasks (e.g. FRAP, FCS)
* BA in life sciences or engineering field
* 5+ years of hands-on experience with light microscopy
* Working knowledge and use of a light microscopes for live cell imaging
* Trained on optics and microscopy technology and theory
* Experience in computer coding (preferably Python)
* Experience developing and following SOPs
* Able to work a flexible schedule
* PhD or Masters in life sciences or engineering field
* Hands on experience with widefield microscopy, spinning disk confocal microscopy, and/or super-resolution microscopy
* Good oral and written communication skills
* Ability to work as part of a team to meet aggressive timelines in a collaborative environment
* Responsible work ethics to ensure adherence to QC standards
Ability to read, analyze, and interpret general business periodicals, professional journals, technical procedures/reports/documents, or governmental regulations. Ability to write reports and business correspondence and speak effectively in front of groups of employees and external populations.
Reasoning and/or critical thinking ability
The ability to define problems, collects data, establishes facts, and draw valid conclusions. Ability to interpret an extensive variety of technical instruction. Constructive thinking and alternative short- to long-term solutions must be considered. Action plans must be developed and implemented.
Please note, this opportunity does not sponsor work visas
It is the policy of the Allen Institute to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by federal, state or local law. In addition, the Allen Institute will provide reasonable accommodations for qualified individuals with disabilities.